DNA
com C

Part:BBa_K233302:Design

Designed by: Amrita Oak, Praveen K Sahu   Group: iGEM09_IBB_Pune   (2009-09-20)


competence factor C , Acinetobacter BD413


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 3665
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 303
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 303
    Illegal NheI site found at 2146
    Illegal SpeI site found at 3666
    Illegal PstI site found at 3680
    Illegal NotI site found at 7
    Illegal NotI site found at 3673
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 303
    Illegal BglII site found at 334
    Illegal BglII site found at 1036
    Illegal BglII site found at 2995
    Illegal BamHI site found at 1900
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 3666
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 303
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 303
    Illegal SpeI site found at 3666
    Illegal PstI site found at 3680
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 745


Design Notes

Normal.


Source

Acinetobacter BD413.

References